Which among the following cell wall components is found only in Gram positive bacteria
Modern physicians frequently prescribe antibiotic medications to help people fight infections. One of the first antibiotics discovered was penicillin. Penicillin was first used to treat bacterial infections in 1942 and is derived from the fungus Penicillium sp. When used as an antibiotic treatment, penicillin operates by a very specific mechanism. Penicillin interferes with the production of a molecule called peptidoglycan. Peptidoglycan molecules form strong links that give the bacterial cell strength as well as preventing leakage from the cytoplasm. Nearly every bacterium has a peptidoglycan cell wall. Show
The composition of the cell wall differs depending on the type of organism, so penicillin does not affect other organisms. The cell walls of plants, for example, are made from cellulose. The cell walls of algae are highly variable. Algae cell walls can be made of cellulose, xylan, silica, carrageenan or a variety of other materials. The cell walls of most fungi are made from chitin. Composition of the cell wall in the archaea is more diverse. Within bacteria, there are two types of bacterial cell walls. Gram-positive bacteria have a peptidoglycan layer on the outside of the cell wall. Gram-negative bacteria have peptidoglycan between membranes. Penicillin works best on gram-positive bacteria by inhibiting peptidoglycan production, making the cells leaky and fragile. The cells burst open and are much easier for the immune system to break down, which helps the sick person heal more quickly. Human cells do not contain peptidoglycan, so penicillin specifically targets bacterial cells.
Other antibiotics target different molecules that inhibit bacterial growth while leaving human cells undamaged. Sulfa antibiotics target a specific enzyme that inhibits bacterial growth. Tetracycline antibiotics bind to bacterial ribosomes that are responsible for protein production and inhibit bacterial protein synthesis. Ciprofloxacin, one of the strongest antibiotics, attacks bacterial DNA replication while leaving human cellular DNA unaffected. Antibiotics are highly specific to a certain bacterial function, and are not helpful for treating non-bacterial illnesses. Viruses are unaffected by antibiotics because they do not have peptidoglycan cell walls or ribosomes, and they do not replicate their own DNA. Bacteria can become resistant to antibiotics through the process of selection and evolution. Penicillin kills most of the bacterial cells, but it does not kill them all. Bacteria resistant to the effects of the antibiotic remain, but in small numbers they can be eliminated from the body by the immune system. It is important to finish all the antibiotics that are prescribed, so that the immune system doesn’t have to work as hard to fight the infection. Both unfinished antibiotic courses and overuse of antibiotics have also led to increased instances of antibiotic resistant bacteria. In bacteriology, gram-positive bacteria are bacteria that give a positive result in the Gram stain test, which is traditionally used to quickly classify bacteria into two broad categories according to their type of cell wall. Gram-positive bacteria take up the crystal violet stain used in the test, and then appear to be purple-coloured when seen through an optical microscope. This is because the thick peptidoglycan layer in the bacterial cell wall retains the stain after it is washed away from the rest of the sample, in the decolorization stage of the test. Conversely, gram-negative bacteria cannot retain the violet stain after the decolorization step; alcohol used in this stage degrades the outer membrane of gram-negative cells, making the cell wall more porous and incapable of retaining the crystal violet stain. Their peptidoglycan layer is much thinner and sandwiched between an inner cell membrane and a bacterial outer membrane, causing them to take up the counterstain (safranin or fuchsine) and appear red or pink. Despite their thicker peptidoglycan layer, gram-positive bacteria are more receptive to certain cell wall targeting antibiotics than gram-negative bacteria, due to the absence of the outer membrane.[1] Characteristics[edit]Gram-positive and gram-negative cell wall structure Structure of gram-positive cell wall In general, the following characteristics are present in gram-positive bacteria:[2]
Only some species have a capsule, usually consisting of polysaccharides. Also, only some species are flagellates, and when they do have flagella, have only two basal body rings to support them, whereas gram-negative have four. Both gram-positive and gram-negative bacteria commonly have a surface layer called an S-layer. In gram-positive bacteria, the S-layer is attached to the peptidoglycan layer. Gram-negative bacteria's S-layer is attached directly to the outer membrane. Specific to gram-positive bacteria is the presence of teichoic acids in the cell wall. Some of these are lipoteichoic acids, which have a lipid component in the cell membrane that can assist in anchoring the peptidoglycan. Classification[edit]Along with cell shape, Gram staining is a rapid method used to differentiate bacterial species. Such staining, together with growth requirement and antibiotic susceptibility testing, and other macroscopic and physiologic tests, forms the full basis for classification and subdivision of the bacteria (e.g., see figure and pre-1990 versions of Bergey's Manual). Species identification hierarchy in clinical settings Historically, the kingdom Monera was divided into four divisions based primarily on Gram staining: Bacillota (positive in staining), Gracilicutes (negative in staining), Mollicutes (neutral in staining) and Mendocutes (variable in staining).[3] Based on 16S ribosomal RNA phylogenetic studies of the late microbiologist Carl Woese and collaborators and colleagues at the University of Illinois, the monophyly of the gram-positive bacteria was challenged,[4] with major implications for the therapeutic and general study of these organisms. Based on molecular studies of the 16S sequences, Woese recognised twelve bacterial phyla. Two of these were gram-positive and were divided on the proportion of the guanine and cytosine content in their DNA. The high G + C phylum was made up of the Actinobacteria and the low G + C phylum contained the Firmicutes.[4] The Actinomycetota include the Corynebacterium, Mycobacterium, Nocardia and Streptomyces genera. The (low G + C) Bacillota, have a 45–60% GC content, but this is lower than that of the Actinomycetota.[2] Importance of the outer cell membrane in bacterial classification[edit]Although bacteria are traditionally divided into two main groups, gram-positive and gram-negative, based on their Gram stain retention property, this classification system is ambiguous as it refers to three distinct aspects (staining result, envelope organization, taxonomic group), which do not necessarily coalesce for some bacterial species.[5][6][7][8] The gram-positive and gram-negative staining response is also not a reliable characteristic as these two kinds of bacteria do not form phylogenetic coherent groups.[5] However, although Gram staining response is an empirical criterion, its basis lies in the marked differences in the ultrastructure and chemical composition of the bacterial cell wall, marked by the absence or presence of an outer lipid membrane.[5][9] All gram-positive bacteria are bounded by a single-unit lipid membrane, and, in general, they contain a thick layer (20–80 nm) of peptidoglycan responsible for retaining the Gram stain. A number of other bacteria—that are bounded by a single membrane, but stain gram-negative due to either lack of the peptidoglycan layer, as in the mycoplasmas, or their inability to retain the Gram stain because of their cell wall composition—also show close relationship to the Gram-positive bacteria. For the bacterial cells bounded by a single cell membrane, the term monoderm bacteria has been proposed.[5][9] In contrast to gram-positive bacteria, all typical gram-negative bacteria are bounded by a cytoplasmic membrane and an outer cell membrane; they contain only a thin layer of peptidoglycan (2–3 nm) between these membranes. The presence of inner and outer cell membranes defines a new compartment in these cells: the periplasmic space or the periplasmic compartment. These bacteria have been designated as diderm bacteria.[5][9] The distinction between the monoderm and diderm bacteria is supported by conserved signature indels in a number of important proteins (viz. DnaK, GroEL).[5][6][9][10] Of these two structurally distinct groups of bacteria, monoderms are indicated to be ancestral. Based upon a number of observations including that the gram-positive bacteria are the major producers of antibiotics and that, in general, gram-negative bacteria are resistant to them, it has been proposed that the outer cell membrane in gram-negative bacteria (diderms) has evolved as a protective mechanism against antibiotic selection pressure.[5][6][9][10] Some bacteria, such as Deinococcus, which stain gram-positive due to the presence of a thick peptidoglycan layer and also possess an outer cell membrane are suggested as intermediates in the transition between monoderm (gram-positive) and diderm (gram-negative) bacteria.[5][10] The diderm bacteria can also be further differentiated between simple diderms lacking lipopolysaccharide, the archetypical diderm bacteria where the outer cell membrane contains lipopolysaccharide, and the diderm bacteria where outer cell membrane is made up of mycolic acid.[7][10][11] Exceptions[edit]In general, gram-positive bacteria are monoderms and have a single lipid bilayer whereas gram-negative bacteria are diderms and have two bilayers. Some taxa lack peptidoglycan (such as the class Mollicutes, some members of the Rickettsiales, and the insect-endosymbionts of the Enterobacteriales) and are gram-variable. This, however, does not always hold true. The Deinococcota have gram-positive stains, although they are structurally similar to gram-negative bacteria with two layers. The Chloroflexota have a single layer, yet (with some exceptions[12]) stain negative.[13] Two related phyla to the Chloroflexi, the TM7 clade and the Ktedonobacteria, are also monoderms.[14][15] Some Bacillota species are not gram-positive. These belong to the class Mollicutes (alternatively considered a class of the phylum Mycoplasmatota), which lack peptidoglycan (gram-indeterminate), and the class Negativicutes, which includes Selenomonas and stain gram-negative.[11] Additionally, a number of bacterial taxa (viz. Negativicutes, Fusobacteriota, Synergistota, and Elusimicrobiota) that are either part of the phylum Bacillota or branch in its proximity are found to possess a diderm cell structure.[8][10][11] However, a conserved signature indel (CSI) in the HSP60 (GroEL) protein distinguishes all traditional phyla of gram-negative bacteria (e.g., Pseudomonadota, Aquificota, Chlamydiota, Bacteroidota, Chlorobiota, "Cyanobacteria", Fibrobacterota, Verrucomicrobiota, Planctomycetota, Spirochaetota, Acidobacteriota, etc.) from these other atypical diderm bacteria, as well as other phyla of monoderm bacteria (e.g., Actinomycetota, Bacillota, Thermotogota, Chloroflexota, etc.).[10] The presence of this CSI in all sequenced species of conventional LPS (lipopolysaccharide)-containing gram-negative bacterial phyla provides evidence that these phyla of bacteria form a monophyletic clade and that no loss of the outer membrane from any species from this group has occurred.[10] Pathogenicity[edit]Colonies of a gram-positive pathogen of the oral cavity, Actinomyces sp. In the classical sense, six gram-positive genera are typically pathogenic in humans. Two of these, Streptococcus and Staphylococcus, are cocci (sphere-shaped). The remaining organisms are bacilli (rod-shaped) and can be subdivided based on their ability to form spores. The non-spore formers are Corynebacterium and Listeria (a coccobacillus), whereas Bacillus and Clostridium produce spores.[16] The spore-forming bacteria can again be divided based on their respiration: Bacillus is a facultative anaerobe, while Clostridium is an obligate anaerobe.[17] Also, Rathybacter, Leifsonia, and Clavibacter are three gram-positive genera that cause plant disease. Gram-positive bacteria are capable of causing serious and sometimes fatal infections in newborn infants.[18] Novel species of clinically relevant gram-positive bacteria also include Catabacter hongkongensis, which is an emerging pathogen belonging to Bacillota.[19] Bacterial transformation[edit]Transformation is one of three processes for horizontal gene transfer, in which exogenous genetic material passes from a donor bacterium to a recipient bacterium, the other two processes being conjugation (transfer of genetic material between two bacterial cells in direct contact) and transduction (injection of donor bacterial DNA by a bacteriophage virus into a recipient host bacterium).[20] In transformation, the genetic material passes through the intervening medium, and uptake is completely dependent on the recipient bacterium.[20] As of 2014 about 80 species of bacteria were known to be capable of transformation, about evenly divided between gram-positive and gram-negative bacteria; the number might be an overestimate since several of the reports are supported by single papers.[20] Transformation among gram-positive bacteria has been studied in medically important species such as Streptococcus pneumoniae, Streptococcus mutans, Staphylococcus aureus and Streptococcus sanguinis and in gram-positive soil bacterium Bacillus subtilis, Bacillus cereus.[21] Orthographic note[edit]The adjectives Gram-positive and Gram-negative derive from the surname of Hans Christian Gram; as eponymous adjectives, their initial letter can be either capital G or lower-case g, depending on which style guide (e.g., that of the CDC), if any, governs the document being written.[22] This is further explained at Gram staining § Orthographic note. 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What cell wall components is found only in gramWall teichoic acids are found only in certain Gram-positive bacteria (such as staphylococci, streptococci, lactobacilli, and Bacillus spp.); so far, they have not been found in gram- negative organisms.
What structures are found in gramGram-positive bacteria are surrounded by many layers of peptidoglycan (PG), which form a protective shell that is 30–100 nm thick (Silhavy et al. 2010). The PG layers are covalently modified with carbohydrate polymers including wall teichoic acids (WTAs) or functionally related anionic glycopolymers as well as CPS.
What are gram positive cell walls made of?The Gram-positive cell wall consists of many interconnected layers of peptidoglycan and lacks an outer membrane. Peptidoglycan prevents osmotic lysis in the hypotonic environment in which most bacteria live. Teichoic acids and lipoteichoic acids are interwoven through the peptidoglycan layers.
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